Advanced Cell Diagnostics

Provider of the most advanced
RNA in situ hybridization technology
Anti-nicastrin monoclonal antibodies elicit pleiotropic anti-tumour pharmacological effects in invasive breast cancer cells., Filipović A, Lombardo Y, Fronato M, Abrahams J, Aboagye E, Nguyen QD, d'Aqua BB, Ridley A, Green A, Rahka E, Ellis I, Recchi C, Przulj N, Sarajlić A, Alattia JR, Fraering P, Deonarain M, Coombes RC., Breast Cancer Res Treat. , 2014 September 24 --- Label-Retaining Cells in the Adult Murine Salivary Glands Possess Characteristics of Adult Progenitor Cells, Chibly AM, Querin L, Harris Z, Limesand KH., PLoS One, 2014 September 19 --- Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina, Stempel AJ, Morgans CW, Stout JT, Appukuttan B., Molecular Vision, 2014 September 19 --- Blood pressure and glucose independent renoprotective effects of dipeptidyl peptidase-4 inhibition in a mouse model of type-2 diabetic nephropathy., Sharkovska Y, Reichetzeder C, Alter M, Tsuprykov O, Bachmann S, Secher T, Klein T, Hocher B., J Hypertens, 2014 September 11 --- Novel RNA Hybridization Method for the In Situ Detection of ETV1, ETV4, and ETV5 Gene Fusions in Prostate Cancer, Kunju LP, Carskadon S, Siddiqui J, Tomlins SA, Chinnaiyan AM, Palanisamy N., Appl Immunohistochem Mol Morphol., 2014 September 09 --- Hyperplasia and fibrosis in mice with conditional loss of the TSC2 tumour suppressor in Müllerian duct mesenchyme-derived myometria, Kaneko-Tarui T, Commandeur AE, Patterson AL, DeKuiper JL, Petillo D, Styer AK, Teixeira JM., Mol Hum Reprod., 2014 September 04 --- Characterization of Inflammatory (Lymphoepithelioma-like) Hepatocellular Carcinoma: A Study of 8 Cases., Patel KR, Liu TC, Vaccharajani N, Chapman WC, Brunt EM., Arch Pathol Lab Med., 2014 September 02 --- Axonally Synthesized ATF4 Transmits a Neurodegenerative Signal across Brain Regions, Baleriola J, Walker CA, Jean YY, Crary JF, Troy CM, Nagy PL, Hengst U., Cell, 2014 August 28 --- The Raccoon Polyomavirus Genome and Tumor Antigen Transcription are Stable and Abundant in Neuroglial Tumors., Brostoff T, Dela Cruz FN Jr, Church ME, Woolard KD, Pesavento PA., J Virol. , 2014 August 27 --- Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos , Gross-Thebing T, Paksa A, Raz E., BioMed Central, 2014 August 15 ---


Delivers Quantitative Molecular Detection and Morphological Context (MD & MC) in a Single Assay

RNAscope® Technology is a cutting-edge multiplex nucleic acid in situ hybridization technology, based on ACD’s unique, patented probe design strategy which enables simultaneous signal amplification and background noise suppression.  RNAscope Technology represents one of the most significant advances in ISH technology in over 40 years.

What is the value of Quantitative Molecular Detection and Morphological Context (MD & MC) in a single assay? What makes RNAscope unique?

  • Molecular Detection: RNAscope detects single RNA molecules and provides quantitative analysis of gene expression at the single-cell resolution.
  • Morphological Context: RNAscope localizes RNA molecules providing spatial and cell-specific expression while preserving tissue architecture.
  • Quantitative: RNAscope quantifies RNA molecules per cell, giving accurate expression level at the single cell resolution across heterogeneous cell populations. 
  • Universal:  RNAscope's patented probe design can be applied to virtually ANY gene in ANY genome in ANY tissue.
  • Scalable: The assay is robust and easy to implement in basic, translational and clinical research labs. Availability of Manual Assays, Automated Assays and Assay Services provide ultimate flexibility.

RNAscope enables in situ detection of RNA biomarkers—filling a long standing gap in in situ molecular analysis.

RNAscope can complement IHC when antibodies are unavailable or have insufficient sensitivity or uncertain specificity. RNAscope is ideal for detecting RNAs for secreted proteins and non-coding RNA—a limitation of IHC.

Downstream of microarrays and NGS (e.g., RNA-seq), RNAscope is an ideal platform for translating new scientific advances into clinical applications due to the speed of RNAscope assay development and its superior sensitivity and specificity.

Other RNA biomarker analysis methods such as RT-PCR can provide quantitative, sensitive and specific measurements of RNA transcripts. However, the resulting quantitative gene expression information is without morphological and cellular context and only represents a “group average” of an admixture of heterogeneous populations of cells. RNAscope’s ability to provide gene expression at the single cell resolution coupled with morphological context is well-suited for RNA analysis in tissue diagnostics applications.

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